96-Well MNT & FISH Assays for Genotoxic Insight
SERVICE INFORMATION
A miniaturised version of a TK6 in vitro micronucleus test in which 2 treatment conditions are utilised in a 96-well microplate format. Micronucleus formation is assessed in 2000 mononuclear cells by microscopy, producing a highly predictive screen of the regulatory OECD 487 endpoint from minimal test item.The study can also be designed to enable incorporation of FISH pan-centromeric staining techniques, to enable additional assessment of micronuclei for presence/absence of centromeres, giving a prediction of whether a material is predominately aneugenic or clastogenic.
Gentronix also offers a version of the 96-well MNT test with automated scoring for faster data turnaround.
Advantages for Screening
– TK6 cell line
– Low compound requirement
– Prediction of OECD 487
– Incorporation of FISH gives prediction of mechanism
| MNT 96-well - Test Cell Line | TK6 |
| Metabolic activation | Typically, induced rat liver S9, though other sources can also be used Short +S9 Long –S9 2 arm study |
| Typical test item requirements | 20 mg (ICH S2(R1)) – < 100 mg (OECD 487) |
| + FISH | < 30 mg |
| Study Duration | < 4 Weeks (TK6 MNT) < 6 Weeks (TK6 MNT + FISH) |
| Endpoint | Micronucleus Formation / Mode of Action |
96-well MNT & FISH
Gentronix offers a range of screening options to help our clients to assess the mutagenic potential of test substances in the Ames test.
SERVICE INFORMATION
Ames assays are bacterial reverse mutation assays that use a variety of Salmonella (or E. coli) strains to detect a range of point mutations and have high sensitivity when used in combination. The Ames test is the most established assay for detecting direct-acting mutagens and forms a key aspect of regulatory genotoxicity testing across industries. As such, an early stage prediction of Ames test outcome is a key step in assessing the toxicological hazard of test substances during development. Gentronix offers a range of service options to meet your project requirements.
Ames 24-well
Conducted in the same full agar plate format as the OECD 471 guideline study, but with multiple options to tailor the study design to meet your requirements. With the help of our team of experts, we can guide you to the most appropriate test strain selection, metabolic activation conditions and study design; delivering a predictive Ames test using a low quantity of test item.