OECD 490: Mouse Lymphoma Assay (MLA)


The mouse lymphoma assay is one of the mammalian gene mutation assays available and is frequently utilised in most genotoxicity testing regulatory frameworks. Able to detect point mutations, clastogens, and in some cases aneugens; the mouse lymphoma assay has an advantage compared to the HPRT (the alternative mammalian gene mutation assay) due to its capability to detect a broader spectrum of genotoxic effects.

The mouse lymphoma assay was previously included in OECD guideline 476 for mammalian cell gene mutation, but was separated out into the new OECD 490 guideline in 2015. Our study director has been working on mouse lymphoma assays for > 14 years, gaining extensive experience of running this study type within industry.

Test systemL5178Y TK+/--3.7.2C
OECD guideline490
Test Format3 hr +/- S9, and extended exposure -S9
Duplicate cultures are used
Typical test item requirementsTypically 5 g
EndpointMammalian gene mutation

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