BlueScreen™ HC utilises a luciferase reporter whilst GreenScreen® HC is based on GFP. The attractive signal-to-noise ratio from the luminescence reporter has made BlueScreen the go-to method. However, the reagent-free detection of GFP means GreenScreen can still be a useful alternative.
Both assays have the advantage of high sensitivity and high specificity compared with the in vitro regulatory battery assays, reducing the number of misleading positives early in compound development. The assays have been extensively validated across a wide range of applicability domains, demonstrating high utility and accuracy for the screening of genotoxic hazard.