Multiflow® Test
SERVICE INFORMATION
Using multiple biomarkers within a single flow cytometry-based experiment, the MultiFlow® assay can distinguish between clastogenic and aneugenic modes of action and identify potentially misleading positives that might be secondary to cytotoxicity. This assay has considerable utility as a follow-up to positive in vitro results or as a primary screening tool, requiring low amounts of compound to generate fast predictions of mechanism and mode of action.
If you would like more details on how Gentronix can support your project by conducting the Multiflow® test, please contact a team member or submit an enquiry to enquiries@gentronix.co.uk.
Advantages for Screening
– High throughput
– Low compound mode of action prediction
– Enables screening for aneugens, clastogens and non-genotoxic substance
| Test Cell Line | TK6 |
| Study Design | Typically, induced rat liver S9, other sources available. Sampling at 4hr & 24hr time points Biomarkers: p53, H2AX, H3, Polyploidy |
| Typical test item requirements | <20 mg |
| Study Duration | < 3 Weeks |
| Endpoint | Multiple biomarkers predict genotoxicity |