OECD 442C Direct Peptide Reactivity Assay Test
SERVICE INFORMATION
The OECD 442C: Direct Peptide Reactivity Assay Test (DPRA) investigates the molecular initiating event of the AOP for skin sensitisation – haptenation. Utilising HPLC, the potential for the reactivity of a test substance to cysteine and lysine peptides is detected. Depletion of these peptides via test substance reactivity is used to support the discrimination between skin sensitisers and non-sensitisers.
DPRA has been validated, and Gentronix has established laboratory proficiency under OECD 442C. Services are available in both GLP and non-GLP formats.
If you would like more details on how Gentronix can support your project by conducting the OECD 442C: Direct Peptide Reactivity Assay Test (DPRA), please contact a team member or submit an enquiry at enquiries@gentronix.co.uk.
OECD guideline | OECD 442C |
Adverse Outcome Pathway Key Event | Number 1 – the molecular initiating event (MIE) |
What is being assessed | Peptide reactivity; the depletion of cysteine and lysine by test substance |
Why is this important | Covalent binding of a substance to skin proteins is considered the first step in skin allergenicity |
Test System | HPLC-UV |
Test Item Requirements | 1 g |
DPRA OECD 442C Proficiency Data
GENTRONIX RESULTS | ||||
---|---|---|---|---|
Test Chemical | in vivo classification | Reactivity Class | DPRA Classification | Classification Correct? |
DNCB | Sensitiser (extreme) | High | Sensitiser | |
Oxazolone | Sensitiser (extreme) | High | Sensitiser | |
Formaldehyde | Sensitiser (strong) | Moderate | Sensitiser | |
Benzyliden acetone | Sensitiser (moderate) | High | Sensitiser | |
Farnesal | Sensitiser (weak) | Moderate | Sensitiser | |
2,3-butanedione | Sensitiser (weak) | High | Sensitiser | |
1-Butanol | Non-sensitiser | No/Minimal | Non-sensitiser | |
6-methyl coumarin | Non-sensitiser | No/Minimal | Non-sensitiser | |
Lactic acid | Non-sensitiser | No/Minimal | Non-sensitiser | |
4-methoxy acetophenone | Non-sensitiser | No/Minimal | Non-sensitiser |