Project Description

Micronucleus Assay 96-Well + FISH

SERVICE INFORMATION

A miniaturised version of a TK6 in vitro micronucleus test in which 2 treatment conditions are utilised in a 96-well microplate format. Micronucleus formation is assessed in 2000 mononuclear cells by microscopy, producing a highly predictive screen of the regulatory OECD 487 endpoint from minimal test item.The study can also be designed to enable incorporation of FISH pan-centromeric staining techniques, to enable additional assessment of micronuclei for presence/absence of centromeres, giving a prediction of whether a material is predominately aneugenic or clastogenic.

Advantages for Screening

– TK6 cell line
– Low compound requirement
– Prediction of OECD 487
– Incorporation of FISH gives prediction of mechanism

MNT 96-well - Test Cell LineTK6
Metabolic activationTypically, induced rat liver S9, though other sources can also be used
Short +S9
Long –S9
2 arm study
Typical test item requirements20 mg (ICH S2(R1)) – < 100 mg (OECD 487)
+ FISH< 30 mg
Study Duration< 4 Weeks (TK6 MNT)
< 6 Weeks (TK6 MNT + FISH)
EndpointMicronucleus Formation / Mode of Action

Predictive Toxicology, Expertly Delivered

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96-well MNT & FISH

Gentronix offers a range of screening options to help our clients to assess the mutagenic potential of test substances in the Ames test.

SERVICE INFORMATION

Ames assays are bacterial reverse mutation assays that use a variety of Salmonella (or E. coli) strains to detect a range of point mutations and have high sensitivity when used in combination. The Ames test is the most established assay for detecting direct-acting mutagens and forms a key aspect of regulatory genotoxicity testing across industries. As such, an early stage prediction of Ames test outcome is a key step in assessing the toxicological hazard of test substances during development. Gentronix offers a range of service options to meet your project requirements.

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Predictive Toxicology, Expertly Delivered

GET IN TOUCH