96-Well MNT and FISH for In Vitro Micronucleus Testing


A miniaturised version of a TK6 in vitro micronucleus test in which 2 treatment conditions are utilised in a 96-well microplate format. Micronucleus formation is assessed in 2000 mononuclear cells by microscopy, producing a highly predictive screen of the regulatory OECD 487 endpoint from minimal test item.The study can also be designed to enable incorporation of FISH pan-centromeric staining techniques, to enable additional assessment of micronuclei for presence/absence of centromeres, giving a prediction of whether a material is predominately aneugenic or clastogenic.

Gentronix also offers a version of the 96-well MNT test with automated scoring for faster data turnaround.

Close-up of a laboratory microscope with a warm lamp in the background, highlighting the precision equipment used in genotoxicity testing.

Advantages for Screening

– TK6 cell line
– Low compound requirement
– Prediction of OECD 487
– Incorporation of FISH gives prediction of mechanism

MNT 96-well - Test Cell LineTK6
Metabolic activationTypically, induced rat liver S9, though other sources can also be used
Short +S9
Long –S9
2 arm study
Typical test item requirements20 mg (ICH S2(R1)) – < 100 mg (OECD 487)
+ FISH< 30 mg
Study Duration< 4 Weeks (TK6 MNT)
< 6 Weeks (TK6 MNT + FISH)
EndpointMicronucleus Formation / Mode of Action

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96-well MNT & FISH

Gentronix offers a range of screening options to help our clients to assess the mutagenic potential of test substances in the Ames test.


Ames assays are bacterial reverse mutation assays that use a variety of Salmonella (or E. coli) strains to detect a range of point mutations and have high sensitivity when used in combination. The Ames test is the most established assay for detecting direct-acting mutagens and forms a key aspect of regulatory genotoxicity testing across industries. As such, an early stage prediction of Ames test outcome is a key step in assessing the toxicological hazard of test substances during development. Gentronix offers a range of service options to meet your project requirements.

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