OECD 487 In Vitro Micronucleus Test
SERVICE INFORMATION
The OECD 487: In vitro Mammalian Cell Micronucleus Test (MNT) is a mainstay of most regulatory genotoxicity testing strategies and is used to detect in vitro clastogens and aneugens. This test evaluates the effects of test substances on genetic material during the stage of cell division, making it a critical tool for identifying potential genotoxicity. In this regard, it complements the Ames test well, as it detects all of the three major classes of genotoxin between the two study types. Fluorescence In Situ Hybridisation (FISH) can also be conducted to differentiate predominantly clastogenic and aneugenic modes of action.
Gentronix has conducted GLP in vitro Mammalian Cell Micronucleus studies since 2014 in isolated human lymphocytes and TK6 cells. Our highly experienced team of scientists has been running, scoring, and study-directing this assay type for many years, ensuring precise and reliable data for evaluating exposure to the test substances.
If you would like more details on how Gentronix can support your project through the conduct of OECD 487: In Vitro Micronucleus Test, please contact a team member or submit an enquiry at enquiries@gentronix.co.uk.
Test systems | TK6 cells, or isolated human lymphocytes |
OECD guideline | 487 |
Metabolic activation | Typically induced rat liver S9, though other sources are available |
Fluorescence In Situ Hybridisation (FISH) inclusion | Available in TK6 cells |
Test Format | 3 hr +/- S9, and extended exposure -S9 Fluorescence microscopy pan-centromeric FISH probes, enable centromeric staining and analysis. |
Typical test item requirements | 200 - 500mg |
Formulation Analysis | Available upon request |
Endpoint | Numerical and structural chromosomal damage |