OECD 471 Ames Test Services
SERVICE INFORMATION
The Ames test, a bacterial reverse mutation assay, is the most commonly requested genotoxicity study in regulatory testing frameworks and has been in use for decades. The test investigates the ability of test substances to cause specific mutations in Salmonella or E. coli strains that allow them to then grow on selective agar plates. Used to detect DNA reactive mutagens, which have the potential to be highly potent in vivo, Ames positive results are taken very seriously when assessing safety.
Gentronix have conducted these studies to GLP since 2014, and our senior study director has been working on Ames tests for over 40 years, meaning we have exceptional experience in this area.
| OECD 471 Ames Test Strains | TA98, TA100, TA1535, TA1537, TA102, TA97, TA97a, uvrA/pKM101, WP2 pKM101, WP2 uvrA |
| Metabolic activation | Typically induced rat liver S9, though other sources are available |
| Test Format | Plate incorporation, pre-incubation |
| Typical test item requirements | 1.5g |
| Formulation Analysis | Available upon request |
| Endpoint | Mutagenicity |