OECD 437 Bovine Corneal Opacity and Permeability Test

SERVICE INFORMATION

The OECD 437: Bovine Corneal Opacity and Permeability Test (BCOP) is an ex vivo method using cows’ eye corneas to determine test chemicals (substances or mixtures) that require classification or labelling in accordance with GHS. Test chemicals are exposed to corneas, enabling categorisation using the LLBO irritancy score.

The OECD 437 BCOP test has been validated, and laboratory proficiency under OECD 437 has been established at Gentronix. Services are available in both GLP and non-GLP formats.

Peira LLBO 180 laser light-based opacitometer used in the OECD 437 BCOP test to measure corneal opacity.
OECD guidelineOECD 437
What is being assessedPotential for eye irritation and serious eye damage and GHS classification and labelling
Why is this importantDetermination of chemicals that require, and those are not require, classification or labelling for eye irritaton and damage is an important aspect of chemical risk assessment and registration.
Test SystemBovine cornea from commercial meat processing
Opacity: light transmission through cornea, quantified using opacimeter
Permeability: contracts dye penetration of corneal layers.
Test Item Requirements 5 g / 5 ml
Scientist wearing a white coat and purple gloves conducting a skin test using a reconstructed human epidermis model.
Memmert incubator used for maintaining controlled environment conditions during skin corrosion tests
Capsule petri dish utilized in the OECD 431 skin corrosion test, highlighting the testing setup.
Peira LLBO 180 laser light-based opacitometer used for bovine corneal examination
Person reviewing a brochure that explains the procedures and importance of the OECD 431 skin corrosion test.

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