Project Description

OECD 442E: h-CLAT

ke3

SERVICE INFORMATION

The OECD 442E human cell activation test (h-CLAT) investigates key event 3 of the skin sensitisation adverse outcome pathway – dendritic cell activation. Utilising the monocytic THP-1 cell line, the ability of a test substance to increase expression of CD54 & CD86 cell surface markers is assessed by flow cytometry. Upregulation of these cell surface markers is associated with dendritic cell activation and leads to the stimulation of an immune response required for sensitisation of the skin to the test substance.

h-CLAT protocols have been validated and laboratory proficiency under OECD 442E established at Gentronix, with services available in both GLP and non-GLP formats.

OECD guidelineOECD 442E
Adverse Outcome Pathway Key EventNumber 3 – activation of dendritic cells
What it measuresIncrease in expression of cell surface markers (CD86 and CD54) measured by flow cytometry
What is the relevance of that?Expression of CD54 & CD86 is linked to the activation of dendritic cells, which is a precursor event for the stimulation of T-cell proliferation, leading to skin allergenicity
Test Systemhuman monocytic leukaemia cell line THP-1
Test Item Requirements 1 g

h-CLAT OECD 442E Proficiency Data

  Gentronix values consistent with OECD reference rangeGentronix study calls consistent with OECD reference
Test chemicalIn vivo classificationCV75CD86CD54CD86 CallCD54 Callh-CLAT classificationClassification correct
2,4-DinitrochlorobenzeneSensitiser (extreme)PositivePositiveSensitiser
4-PhenylenediamineSensitiser (strong)PositiveNegativeSensitiser
Nickel sulfateSensitiser (moderate)PositivePositiveSensitiser
2-MercaptbenzothiazoleSensitiser (moderate)NegativePositiveSensitiser
R(+)-LimoneneSensitiser (weak) Negative PositiveSensitiser
Imidazolidinyl ureaSensitiser (weak) Positive PositiveSensitiser
IsopropanolNon-sensitiser Negative NegativeNon-sensitiser
GlycerolNon-sensitiser Negative NegativeNon-sensitiser
Lactic acidNon-sensitiser Negative NegativeNon-sensitiser
4-Aminobenzoic acidNon-sensitiser Negative NegativeNon-sensitiser

Predictive Toxicology, Expertly Delivered

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