Project Description

OECD 487: In vitro MNT


The in vitro micronucleus test (MNT) is a main-stay of most regulatory genotoxicity testing strategies, and is used to detect in vitro clastogens and aneugens.  In this regard it complements the Ames test well, as between the two study types they detect all of the three major classes of genotoxin. Fluorescence In Situ Hybridisation (FISH) can also be conducted to differentiate predominantly clastogenic and aneugenic modes of action.

We have been conducting GLP in vitro MNT studies since 2014, in both isolated human lymphocytes and in TK6 cells.  We have a highly experienced team of scientists that have been running, scoring and study directing this assay type for many years.

Test systemsTK6 cells, or isolated human lymphocytes
OECD guideline487
Metabolic activationTypically induced rat liver S9, though other sources are available
Fluorescence In Situ Hybridisation (FISH) inclusionAvailable in TK6 cells
Test Format3 hr +/- S9, and extended exposure -S9
Fluorescence microscopy pan-centromeric FISH probes, enable centromeric staining and analysis.
Typical test item requirements1 - 1.5g
Formulation AnalysisAvailable upon request
EndpointNumerical and structural chromosomal damage
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