OECD 431: In Vitro Skin Corrosion using Reconstructed Human Epidermis (RhE)

SERVICE INFORMATION

Testing for skin corrosivity is an important part of safety profiling to avoid serious harm to chemical users or consumers. The in vitro OECD 431 skin corrosion test in reconstructed human epidermis can form part of an animal free approach to hazard identification for safety purposes and meeting classification and labelling requirements. This can be part of a top-down or bottom-up approach in conjunction with skin irritation tests.

Skin corrosion testing has been validated and laboratory proficiency under OECD 431 established at Gentronix, with services available in both GLP and non-GLP formats.

We offer a high capacity , exceptional quality and rapid turnaround times to me our clients need.

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For innovative solutions in both genetic toxicology and skin toxicology, contact us and see how we can help.

Or contact us on info@gentronix.co.uk or +44 (0) 1625 238700

MORE ABOUT OECD 431

For further details on how Gentronix can support the risk assessment of your materials, please take a look at our GLP Skin toxicology services.

OECD guidelineOECD 431
What is being assessedTest substance induced corrosion and severe damage to skin
Why is this importantIdentification of substances with skin corrosion potential is an important aspect of chemical risk assessment and hazard classification
Test SystemMatTek EpiDerm™ reconstituted human epidermis
Cytotoxicity measured using MTT
Test Item Requirements5 g / 5 ml
Preliminary TestingAssessment for MTT reducers; Test Chemical Interference; Mesh Reactivity

Test Chemicalin vivo classification (OECD 404)*Physical StateOECD 431 Expected Results*Gentronix Classification Correct?
Bromoacetic acidCategory 1AOrganic acidCategory 1A
Boron trifluoride dihydrateCategory 1AInorganic acidCategory 1A
PhenolCategory 1APhenolCategory 1A
Dichloroacetyl chlorideCategory 1AElectrophileCategory 1A
Glyoxylic acid monohydrateCategory 1B and 1COrganic acidCategory 1B and 1C
Lactic acidCategory 1B and 1COrganic acidCategory 1B and 1C
Hydrochloric acid (14.4%)Category 1B and 1CInorganic acidCategory 1B and 1C
Phenethyl bromide None CorrosiveElectrophile None Corrosive
4-Amino1,2,4- triazole None CorrosiveOrganic base None Corrosive
4-(methylthio)- benzaldehyde None CorrosiveElectrophile None Corrosive
Lauric acid None CorrosiveOrganic acid None Corrosive
*reported from OECD guideline 431

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SKIN & OCULAR SAFETY SUPPORTING COSMETIC REGULATORY TESTING AS NON-ANIMAL ALTERNATIVES METHODS

Skin Toxicology
OECD 431 Skin Corrosion OECD 439 Skin Irritation

Ocular Toxicology
OECD 492 EpiOcular OECD 437 BCOP

Skin Sensitisation
OECD 442C DPRA OECD 442D KeratinoSensTM OECD 442E h-CLAT